In this paper, we experimentally verify the antibunching properties of multiple single photons located at distinct optical modes in a quantum frequency comb generated by cavity-enhanced spontaneous four-wave mixing in an InP membrane micro-ring resonator. The experimentally measured single photon conditional self-correlation parameter ${{\boldsymbol{g}}}_{{\boldsymbol{H}}}^{\left(2\right)}\left(0\right)=0.08\,\pm \,0.008,$ well below the classical regime (0.5) with the corresponding detector corrected Klyshko efficiency η k of 3%–4.2%.